DNABarcodeCompatibility - A Tool for Optimizing Combinations of DNA Barcodes Used in
Multiplexed Experiments on Next Generation Sequencing Platforms
The package allows one to obtain optimised combinations of
DNA barcodes to be used for multiplex sequencing. In each
barcode combination, barcodes are pooled with respect to
Illumina chemistry constraints. Combinations can be filtered to
keep those that are robust against substitution and
insertion/deletion errors thereby facilitating the
demultiplexing step. In addition, the package provides an
optimiser function to further favor the selection of barcode
combinations with least heterogeneity in barcode usage.